In SaxoCell CAR-NK 4.0, we are interested in improving the quality of NK-cells after cryopreservation. In the field, different freezing approaches were tested. However, there seems to be a potential for improvement. Since, I am new in the field, I was wondering, wether there is a common sence on freezing protocols? Do you have any recommendations, which parameters (cryoprotectants, concentration of DMSO, additives, CRF,… ) could be testet or which mistakes we can avoid by your piece of advice?
I am grateful for any tips, you can share! 🙂